Effect of Serine Hydroxamate and Methyl a-D-glucopyranoside Treatment on Nucleoside Polyphosphate Pools, RNA and Protein Accumulation in Streptomyces hygroscopicus

1 Akademie der Wissenschaften der DDR. Forschungszentrum Jur Molekularbiologie und Medizin, Zentralinstitut fur Mikrobiologie und experimentelle Therapie, Beutenbergstrasse It, Schliessfach 73. DDR-6900 Jena. GDR 2 Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, POB 521. Hungary ABSTRACT SUMMARY: The accumulation of RNA and protein and the kinetics of nucleoside triphosphate and guanosine polyphosphate pools during amino acid starvation and carbon source downshift were investigated in Streptomyces hygroscopicus. RNA accumulation was controlled stringently during both amino acid starvation and carbon source downshift. The pool size of ppGpp increased dramatically under these conditions. However, the intracellular concentrations of nucleoside triphosphates were low and the concentration of guanosine polyphosphates was much lower than in Escherichia coli. The possible significance of this phenomenon in the regulation is discussed.