Agrococcus citreus sp. nov., isolated from a medieval wall painting of the chapel of Castle Herberstein (Austria)

1 Institut für Mikrobiologie und Genetik, Universität Wien, A-1030 Wien, Austria 2 Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria 3 DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Aussenstelle Jena, Germany 4 Hans-Knöll-Institut für Naturstoff-Forschung e. V., D-07745 Jena, Germany 5 DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, D-38124 Braunschweig, Germany Author for correspondence: Hans-Jürgen Busse. Tel: +43 1 25077 2119. Fax: +43 1 25077 2190. e-mail: Hans-Juergen.Busse{at}vu-wien.ac.at ABSTRACT A bacterial strain, D-1/1a T , isolated from a medieval wall painting of the chape of Herberstein (Styria, Austria) was characterized by a polyphasic approach. Strain D-1/1a T shared 98·1% 16S rRNA sequence similarity to Agrococcus jenensis . The chemotaxonomic characteristics including polar lipid pattern, whole cell sugars, quinone system, polyamine pattern, cell wall composition and fatty acid profile were in good agreement with those of Agrococcus jenensis . The G+C content of the DNA was determined to be 74 mol%. The value of 47% DNA reassociation obtained after DNA-DNA hybridization between DNA of Agrococcus jenensis and strain D-1/1a T as well as differences in the amino acid composition of the peptidoglycan and in physiological characteristics demonstrate that the isolate represents a new species of the genus Agrococcus . The name Agrococcus citreus sp. nov. is proposed for the new species harbouring isolate D-1/1a T . The type strain is DSM 12453 T . Key Words: Agrococcus citreus sp. nov. • 16S rDNA sequence analysis • chemotaxonomy • physiology • DNA reassociation The EMBL accession number for the 16S rDNA sequence of strain D-1/1a T is AJ012826 .