Vascular Influences of Calcium Supplementation and Vitamin D–Induced Hypercalcemia in NaCl-Hypertensive Rats

This 8-week study investigated the effects of increasing dietary Ca content from 1.0% to 3.0% and hypercalcemia induced by oral 1α-OH vitamin D3 (1OH-D3, 1.2 μg/kg), on arterial tone in NaCl-hypertensive rats. The high-Ca diet completely prevented the increase in blood pressure induced by the 6.0% NaCl chow, while plasma total Ca and body weight were not different from controls. The 1OH-D3 treatment moderately elevated plasma total Ca and attenuated the NaCl-induced rise in blood pressure, but also impaired weight gain. The tone of isolated mesenteric arterial rings was examined at the end of study. The endothelium-independent relaxations to nitroprusside, isoproterenol, and cromakalim were impaired in NaCl-hypertension. Experiments with N-nitro-l-arginine methyl ester and tetraethylammonium in vitro suggested that both the nitric oxide- and hyperpolarization-mediated components of endothelium-dependent relaxation to acetylcholine were reduced in NaCl-hypertensive rats. All of the impaired relaxations in NaCl hypertension were normalized by concomitant Ca supplementation. The 1OH-D3 treatment did not affect vascular relaxation, but it attenuated maximal contractile responses induced by norepinephrine and KCl by more than 50%. The reduced vasoconstrictor responses could not be explained by increased apoptosis in the vessel wall, but calcification may have played a role, since moderate signs of medial or adventitial calcification were observed in the aortic preparations after the 1OH-D3 treatment. In conclusion, a high-Ca diet, which did not cause hypercalcemia, normalized blood pressure and endothelium-dependent and endothelium-independent vasorelaxation in NaCl-hypertensive rats. In contrast, chronic hypercalcemia induced by 1OH-D3 was associated with moderately lowered blood pressure, possibly because of reduced vasoconstrictor responses in arterial smooth muscle.