Experimental Oral Hepatography in Rats

Transitory opacification of the rat liver is possible by oral administration of an emulsion of iodinated poppyseed oil (Lipiodol). Investigation of several variables affecting liver opacification indicated that hepatography could be achieved most efficiently when multiple doses of the contrast material were given to fasting young animals. Although the diagnostic liver opacification produced in most animals was acceptable, fatality frequently resulted from iodine toxicity. Histologic examination was conducted on animals that died to determine the tissue manifestations of the toxicity. Pharmacodynamic studies established the amount of Lipiodol in the liver and gastrointestinal tract at intervals after its administration. The deposition of 27 mg of Lipiodol (11 mg iodine) per gram of rat liver was determined as the amount necessary to achieve diagnostic opacification.