Toxicity of heat sterilized peritoneal dialysis fluids is derived from degradation of glucose

Heat sterilization makes peritoneal dialysis (PD) solutions cytotoxic. Two compounds in the solutions, lactate and glucose, can be degraded by heat. This study's goal was to discover which of the compounds was responsible for the cytotoxicity. The influence of sterilization temperature on degradation of the compounds was also subjected to investigation. Solutions of glucose and lactate and a mixture of lactate and glucose were prepared. These were sterilized in glass ampules in an oil bath at different temperatures for varying times. Toxicity was determined as inhibition of cell growth with a fibroblast cell line (L929), and ultraviolet (UV) absorbance was measured at 284 nm. Lactate solutions did not show cytotoxicity after heat sterilization. Glucose solutions that were heat sterilized showed an increase in UV absorbance at 284 nm and were cytotoxic. The mixture of lactate and glucose exhibited the same cytotoxicity as glucose alone. Lower sterilization temperatures lead to increased cytotoxicity and an increase in UV absorbance at 284 nm. Results indicate that the toxic products formed during heat sterilization of PD fluids are derived from glucose.