rapid microassay for determination of peroxidase in wheat and flour

A rapid sensitive microplate assay for the determination of peroxidase activity in wheat, flour, or individual wheat kernels has been developed using a commercially prepared 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) solution. The assay derives a six-point calibration curve based on commercially available horseradish peroxidase (r2 > 0.990), while simultaneously analyzing eight extracts in triplicate. The coefficients of variation (CV) of triplicate assays of a single extract from any of the three sources investigated were generally