Influence of benzimidazole on callus growth and shoot formation on callus cultures

The influence of benzimidazole on callus growth and shoot formation in vitro was examined. At concentrations of 8.5 × 10−4mM to 8.5 × 10−1mM benzimidazole failed to stimulate callus growth or shoot formation. Concentrations of 0.85 mM and 1.7 mM inhibited kinetin-dependent callus growth while lower concentrations were without effect. It may be inferred that benzimidazole has no cell division properties such as is associated with the cytokinins, though it mimics, in appearance, cytokinins actions in several physiological processes.