Immunocytochemistry of rhamnogalacturonan II in cell walls of higher plants

A polyclonal antibody against a borate-RG-II complex is raised in rabbits. The antibody recognized RG-II exclusively in cell wall polysaccharides. Immunocytochemical studies demonstrated that the epitope is ubiquitous in cell walls of all the cells in radish and rice roots cultured tobacco cells, re...

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Veröffentlicht in:Plant and cell physiology 1998-05, Vol.39 (5), p.483-491
Hauptverfasser: Matoh, T. (Kyoto Univ. (Japan)), Takasaki, M, Takabe, K, Kobayashi, M
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Sprache:eng
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Zusammenfassung:A polyclonal antibody against a borate-RG-II complex is raised in rabbits. The antibody recognized RG-II exclusively in cell wall polysaccharides. Immunocytochemical studies demonstrated that the epitope is ubiquitous in cell walls of all the cells in radish and rice roots cultured tobacco cells, red clover root nodules, and lily growing pollen tubes. The label was denser in proximal to plasma membrane, and not detected in middle lamella, suggesting that borate may cross-like newly secreted pectic polysaccharides at the membrane-cell wall interface
ISSN:0032-0781
1471-9053
DOI:10.1093/oxfordjournals.pcp.a029395