Phosphoenolpyruvate carboxylase of maize [Zea mays] leaves: An improved method for purification and reduction of the inhibitory effect of malate by ethylene glycol and bicarbonate

Light-adapted and dark-adapted forms of phosphoenolpyruvate carboxylase were purified from maize leaves by an improved method that included chromatography on Butyl-Toyopearl in the presence of ethylene glycol. The inhibition by malate was relieved not only by increasing concentrations of ethylene gl...

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Veröffentlicht in:	Plant and cell physiology 1997, Vol.38 (1), p.76-80
Hauptverfasser:	Ogawa, N. (Tokyo Univ. (Japan). Faculty of Agriculture), Kai, T, Yabuta, N, Izui, K
Format:	Artikel
Sprache:	eng
Schlagworte:	ALCOHOLES ALCOHOLS ALCOOL BICARBONATE BICARBONATES BICARBONATOS Biological and medical sciences C4 photosynthesis C4photosynthesis dark dose response Enzymes ETHYLENE Ethylene glycol ETILENO FEUILLE FOSFENOLPIRUVATO CARBOXILASA Fundamental and applied biological sciences. Psychology HOJAS INHIBICION INHIBITION LEAVES light MALATE Malate inhibition MALATES MALATOS malic acid Metabolism methodology PHOSPHOENOLPYRUVATE CARBOXYLASE Phosphoenolpyruvate carboxylase (EC 4.1.1.31) photosynthesis Plant physiology and development protein composition protein content PURIFICACION PURIFICATION REDUCCION REDUCTION ZEA MAYS
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Beschreibung
Zusammenfassung:	Light-adapted and dark-adapted forms of phosphoenolpyruvate carboxylase were purified from maize leaves by an improved method that included chromatography on Butyl-Toyopearl in the presence of ethylene glycol. The inhibition by malate was relieved not only by increasing concentrations of ethylene glycol but also by bicarbonate at pH 7.0.
ISSN:	0032-0781 1471-9053
DOI:	10.1093/oxfordjournals.pcp.a029088