Physiology: Human follicular fluid maturity and endothelial cell mitogenesis
Follicular fluid, of varying maturity, (day 5–16 of cycle) was collected from the largest Graafian follicle of each of 22 ovulatory patients during laparoscopic procedures. Three samples were blood-stained and discarded. The mitogenic potential of each sample was determined using bovine aortic endot...
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Veröffentlicht in: | Human reproduction (Oxford) 1993-10, Vol.8 (10), p.1564-1569 |
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Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Follicular fluid, of varying maturity, (day 5–16 of cycle) was collected from the largest Graafian follicle of each of 22 ovulatory patients during laparoscopic procedures. Three samples were blood-stained and discarded. The mitogenic potential of each sample was determined using bovine aortic endothelial cells in the CellTiter 96TM Non-Radioactive Cell Proliferation/Cytotoxicity Assay system. Intra- and inter-plate coefficients of variation were |
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ISSN: | 0268-1161 1460-2350 |
DOI: | 10.1093/oxfordjournals.humrep.a137892 |