Physiology: Human follicular fluid maturity and endothelial cell mitogenesis

Follicular fluid, of varying maturity, (day 5–16 of cycle) was collected from the largest Graafian follicle of each of 22 ovulatory patients during laparoscopic procedures. Three samples were blood-stained and discarded. The mitogenic potential of each sample was determined using bovine aortic endot...

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Veröffentlicht in:Human reproduction (Oxford) 1993-10, Vol.8 (10), p.1564-1569
Hauptverfasser: McClure, N., Macpherson, A.M., Abberton, K.M., Healy, D.L., Rogers, P.A.W.
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Sprache:eng
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Zusammenfassung:Follicular fluid, of varying maturity, (day 5–16 of cycle) was collected from the largest Graafian follicle of each of 22 ovulatory patients during laparoscopic procedures. Three samples were blood-stained and discarded. The mitogenic potential of each sample was determined using bovine aortic endothelial cells in the CellTiter 96TM Non-Radioactive Cell Proliferation/Cytotoxicity Assay system. Intra- and inter-plate coefficients of variation were
ISSN:0268-1161
1460-2350
DOI:10.1093/oxfordjournals.humrep.a137892