Characterization of the ‘In Vivo’ Nitrate Reductase Activity in the Roots and Leaves of Eichhornia crassipes

Characterization of the ‘In Vivo’ Nitrate Reductase Activity in the Roots and Leaves of Eichhornia crassipes

Studies of in vivo nitrate reductase activity (NRA) in Eichhornia crassipes showed that adding 5% isopropanol gave highest NRA in all parts of the plant, but it was also necessary to flush the assay media containing leaves with N1 to obtain the maximal activity. There were differences in the pH opti...

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Veröffentlicht in:Annals of botany 1990-05, Vol.65 (5), p.525-528
1. Verfasser: WIGNARAJAH, K.
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Eichhornia crassipes
Enzymes
Fundamental and applied biological sciences. Psychology
Kinetics
Leaves
Metabolism
Nitrate reductase activity
Nitrates
Nitrites
Petioles
Plant physiology
Plant physiology and development
Plant roots
Plants
Soybeans
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Zusammenfassung:Studies of in vivo nitrate reductase activity (NRA) in Eichhornia crassipes showed that adding 5% isopropanol gave highest NRA in all parts of the plant, but it was also necessary to flush the assay media containing leaves with N1 to obtain the maximal activity. There were differences in the pH optima for NRA, the root optimum being 6·5 leaf 7·5–8·0, and the petiole showing a major peak at 6·5 and a minor peak at 7·.5–8·0. Possible significances of these differences are discussed.
ISSN:0305-7364
1095-8290
DOI:10.1093/oxfordjournals.aob.a087964