A photochemical detection of methylcytosine by using hydrophobic interaction

Abstract In this paper, the nonenzymatic detection of 5-methylcytosine with high selectivity is described. We present a new methylation detection method by using template-directed photoligation through 5-cyanovinyl-2′-deoxyuridine (CU). Significantly, the photoligation yield of the 5-methylcytosine...

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Veröffentlicht in:	Nucleic Acids Symposium Series 2009, Vol.53 (1), p.203-204
Hauptverfasser:	Ami, Takehiro, Ogino, Masayuki, Taya, Yuuta, Takemura, Yumiko, Fujimoto, Kenzo
Format:	Artikel
Sprache:	eng
Schlagworte:	5-Methylcytosine - analysis 5-Methylcytosine - chemistry Deoxyuridine - analogs & derivatives Deoxyuridine - chemistry Hydrophobic and Hydrophilic Interactions Oligodeoxyribonucleotides - chemistry Photochemical Processes Photochemistry - methods
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Zusammenfassung:	Abstract In this paper, the nonenzymatic detection of 5-methylcytosine with high selectivity is described. We present a new methylation detection method by using template-directed photoligation through 5-cyanovinyl-2′-deoxyuridine (CU). Significantly, the photoligation yield of the 5-methylcytosine case was approximately 9.1-fold higher than that in the case of cytosine.
ISSN:	0261-3166 1746-8272
DOI:	10.1093/nass/nrp102