anatomy of the tRNA-rRNA region of the Neurospora crassa mitochondrial DNA

To obtain more information about the arrnangement of Hind III restriction fragmengnts in the tRNA-rRNR region of the Neuroaspora crassa a mitochondrial (mt) DNA we have cleaved the mtDNA with Rpa I and Hind II. We could construct additional cleavage maps for these enzymes. Hybridization of rRNAs to Hind 11 fragments confiried the existence of an intervening region of about 2,500 basepairs in the 24S rRNA (Hahn et al., Cell, in press). About seven tRNA genes, among which the genea for tRNA1Ser and tRNAMMet, are located in a segment of about 5,000 bp separating the 245 and 17S rRNA genes. Another cluster of 14 tRNA gems is found adjust to the other end of the 24s gene. The gense for tRNA1Leu and tRNAFMet are located in this cluster.