Immunoglobulin genes of the x light chain type from two human lymphoid cell lines are closely related
As a first step in our studies of functionally rearranged K genes of man we cloned the germline JK-CK region from placenta DNA employing a mouse JK clone as hybridization probe. Subclones of the human JK-CK region were then used to characterize and clone the rearranged K genes of the lymphoid cell l...
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Veröffentlicht in: | Nucleic acids research 1984-09, Vol.12 (18), p.6995-7006 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | As a first step in our studies of functionally rearranged K genes of man we cloned the germline JK-CK region from placenta DNA employing a mouse JK clone as hybridization probe. Subclones of the human JK-CK region were then used to characterize and clone the rearranged K genes of the lymphoid cell lines Walker and Daudi. The Walker cell line contains one rearranged and one germline K allele (K+,KO; ref. 1). Only one K gene was found in Daudi cells (K+). Restriction mapping and DNA sequencing showed, that the rearranged K genes from both cell lines are closely related. These features make the two cell lines particularly suitable for studies on the chromatin structure of K light chain genes. The 5′ flanks of the two genes (388 bp) are identical while there is a 12 % divergence between the VK gene segments themselves. This situation may reflect somatic mutation processes and/or gene conversion like events. |
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ISSN: | 0305-1048 1362-4962 |
DOI: | 10.1093/nar/12.18.6995 |