High Efficiency Plant Regeneration from Protoplasts of the Indica Cultivar IR58

Procedures have been established for embryogenic cell suspension, protoplast culture, and plant regeneration from the Indica rice cultivar, IR58. Embryogenic cell suspension was established in modified R2 medium and maintained in amino acid-based AA medium. Protoplasts were cultured in R2 basal medi...

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Veröffentlicht in:	Journal of experimental botany 1992-12, Vol.43 (12), p.1633-1637
Hauptverfasser:	TORRIZO, L. B., ZAPATA, F J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology Callus Cell culture techniques Cell lines Embryonic cells Fundamental and applied biological sciences. Psychology Indica Maltose Nurses Plant physiology and development Plants Protoplast Protoplast culture Protoplasts Regeneration Rice Rice (Oryza sativa Tissue cultures, protoplasts
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Beschreibung
Zusammenfassung:	Procedures have been established for embryogenic cell suspension, protoplast culture, and plant regeneration from the Indica rice cultivar, IR58. Embryogenic cell suspension was established in modified R2 medium and maintained in amino acid-based AA medium. Protoplasts were cultured in R2 basal medium containing B5 vitamins. Plants were regenerated in MS basal medium with 3% maltose as carbon source. Selection of embryogenic protoplast-derived calli is an important step for high-frequency plant regeneration. More than 900 plants were regenerated through this procedure.
ISSN:	0022-0957 1460-2431
DOI:	10.1093/jxb/43.12.1633