Isolation and characterization of a carrot cell line resistant to methotrexate

A carrot cell line (WCA1) resistant to Mtx has been isolated and partially characterized. The IC50-Mtx is about thirty times higher in the resistant than in the parental line and the rate of uptake of Mtx is decreased 15 times. In addition the specific activity of DHFR is about three times higher in...

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Veröffentlicht in:	Journal of experimental botany 1984-09, Vol.35 (158), p.1390-1397
Hauptverfasser:	Cella, R, Albani, D, Biasini, M.G, Carbonera, D, Parisi, B
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions B lymphocytes Biochemistry Biological and medical sciences carrots Cell culture techniques Cell extracts Cell lines Chromosomes Classical genetics, quantitative genetics, hybrids Cultured cells Daucus carota Dihydrofolate reductase Economic plant physiology Enzymes Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution In vitro culture inhibitors methotrexate Methotrexate resistance Methotrexate uptake Plant cell suspension culture Plant cells Pteridophyta, spermatophyta Somatic cells Vegetals
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Zusammenfassung:	A carrot cell line (WCA1) resistant to Mtx has been isolated and partially characterized. The IC50-Mtx is about thirty times higher in the resistant than in the parental line and the rate of uptake of Mtx is decreased 15 times. In addition the specific activity of DHFR is about three times higher in the resistant line as compared to the parent
ISSN:	0022-0957 1460-2431
DOI:	10.1093/jxb/35.9.1390