Comparison of the reveal 20-hour method and the BAM culture method for the detection of Escherichia coli O157:H7 in selected foods and environmental swabs : Collaborative study

Four different food types along with environmental swabs were analyzed by the Reveal for E. coli O157:H7 test (Reveal) and the Bacteriological Analytical Manual (BAM) culture method for the presence of Escherichia coli O157:H7. Twenty-seven laboratories representing academia and private industry in...

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Veröffentlicht in:	Journal of AOAC International 2001-05, Vol.84 (3), p.737-751
Hauptverfasser:	BIRD, Charles B, HOERNER, Rebecca J, RESTAINO, Lawrence
Format:	Artikel
Sprache:	eng
Schlagworte:	Beverages - microbiology Biological and medical sciences Culture Media Environmental Microbiology - standards Escherichia coli O157 - growth & development Escherichia coli O157 - isolation & purification Food industries Food microbiology Food Microbiology - standards Fruit Fundamental and applied biological sciences. Psychology General aspects Immunosorbent Techniques Laboratories Lactuca - microbiology Methods of analysis, processing and quality control, regulation, standards Microbiological Techniques
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Zusammenfassung:	Four different food types along with environmental swabs were analyzed by the Reveal for E. coli O157:H7 test (Reveal) and the Bacteriological Analytical Manual (BAM) culture method for the presence of Escherichia coli O157:H7. Twenty-seven laboratories representing academia and private industry in the United States and Canada participated. Sample types were inoculated with E. coli O157:H7 at 2 different levels. Of the 1,095 samples and controls analyzed and confirmed, 459 were positive and 557 were negative by both methods. No statistical differences (p
ISSN:	1060-3271 1944-7922
DOI:	10.1093/jaoac/84.3.737