Studies in improvement of Official Method 996.06

Quantitation of fat in foods has been performed successfully with AOAC Official Method 996.06. A number of situations have been encountered that render the method note, "Note: For any unknown or uncalibrated peaks, use the nearest calibrated fatty acid response factors and conversion factors," inaccurate. Identification of extraneous compounds and availability of additional standard fatty acid methyl esters combined with mass spectral data lead to the recommendation of modifications in Official Method 996.06. The stepwise performance of the method remains unchanged.