Quantitation of Mycotoxins in Four Food Matrices Comparing Stable Isotope Dilution Assay (SIDA) with Matrix-Matched Calibration Methods by LC–MS/MS

Background: Mycotoxins are big concerns in food safety. Analytical methods are important for the evaluation of mycotoxins in different food commodities. Objective: In this study, stable isotope dilution assay (SIDA) was compared with a matrix-matched calibration method for the quantification of mycotoxins in four different commercially available commodities and two reference materials. Methods: All samples were extracted with water–acetonitrile (50+50, v/v), followed by filtration and LC–tandem MS analysis. Results: SIDA calibration accuracies ranged from 78.6 to 112% with relative SDs (RSDs) ≤16% across all four matrices. The majority of the recoveries across all matrices ranged from 70 to 120% with RSDs 90%, whereas accuracies for the mismatched mycotoxin/13C-IS were