Studies on the nutrition and metabolism of animal cells in serum-free media: II. Cultivation of cells in suspension

Studies on the nutrition and metabolism of animal cells in serum-free media: II. Cultivation of cells in suspension

A serum-free system for the growth of a variety of animal cells in suspension is presented. Cell populations exceeding 4 X 10⁶ per ml have been obtained for L cells, and growth comparable to that obtained in serum-containing medium is reported for other cell lines. The stimulatory effect of insulin...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The Journal of infectious diseases 1963-05, Vol.112 (3), p.221-225
Hauptverfasser: Tribble, Henry R., Higuchi, Kiyoshi
Format: Artikel
Sprache:eng
Schlagworte:
Animal cells
Cell culture techniques
Cell growth
Cell lines
Culture Media
Culture Media, Serum-Free
Cultured cells
Insulin
Kidney cells
L cells
Nutritional Status
Research Design
Spleen cells
T lymphocytes
Tissue Culture Techniques
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A serum-free system for the growth of a variety of animal cells in suspension is presented. Cell populations exceeding 4 X 10⁶ per ml have been obtained for L cells, and growth comparable to that obtained in serum-containing medium is reported for other cell lines. The stimulatory effect of insulin and the requirementfor methocel by most cells were demonstrated.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/112.3.221