P-293 Euploidy results through niPGT techniques

Abstract Study question Which are the euploid results using niPGT? Summary answer Our niPGT (non invasive PGT) results are similar to the one obtained through traditional PGT-A What is known already Recent studies have shown the presence of embryo cell-free DNA (cfDNA ) in spent blastocyst medium (SBM), opening a new era of possibilities for non-invasive preimplantation genetic testing for aneuploidy (niPGT-A). Study design, size, duration We performed a retrospective study including 80 patients of IVF between August 2020 and December 2021, among them, 60 used their own egg, and 20 donor eggs. 237 embryos were analysed through niPGT. Participants/materials, setting, methods Embryos were cultured in a Geri incubator (Merck) up to day 4, and then individually cultured in 10 µl drops of CCSS (Fujifilm) until day 6 in a bench-top Minc (Cook). At day 6, after at least 40 hours in culture, blastocysts were vitrified and medium collected in sterile PCR tubes. After collection, medium was immediately frozen and sent for sequencing to our reference laboratory (Igenomix, Spain). Main results and the role of chance Euploidy ratio by niPGT analysis was significantly lower in patients older than 40 years (15%) comparing with both 35-40 year patient (48%) and younger than 35 years old patients (50%) (p