P-210 Development of a new device for automatic vitrification of oocytes

Abstract Study question Could an automatic vitrification device prevent human errors and lead to better results and the same survival rates to avoid variability between reproductive centres? Summary answer Developing an automatic vitrification device using Kitazato vitrification/warming medium could optimize survival rates and outcome, regardless of the centre or user using the technique. What is known already Vitrification is the most common technique used daily in assisted reproduction clinics for oocytes and embryos cryopreservation. This procedure is becoming more popular since women are delaying motherhood and they want to preserve their fertility. The most used methodology worldwide is the Kitazato protocol, which has reported the best survival rates (>90%). Although these results are attractive, the procedure involves several manual steps, which requires highly skilled and trained embryologists to be as reproducible as possible. It exists variability between users according to their experience and between clinics so, a device that ensures reproducibility, safety and efficacy is the future. Study design, size, duration The automated device and the microfluidic chip were designed by our team. During 3 weeks, 210 mice zygotes were used in the study to mimic oocyte behaviour because of their greater availability, similar features and their use for trials of biocompatibility of materials for subsequent use in human samples. They were divided in 2 groups to compare manual and automated vitrification using Kitazato media. Both groups were warmed manually according to standard Kitazato protocol. Participants/materials, setting, methods Mice zygotes (210) were divided in 2 groups: A) Manual control (n = 103) with standard vitrification protocol by Kitazato; B) Automated group (n = 107), following a microfluidic protocol performed with Kitazato media. All the zygotes were stored with the same vitrification device: Cryotop by Kitazato. After their storage in liquid nitrogen, both groups were warmed manually following the standard Kitazato thawing protocol. Chi square test was performed to compare survival and blastocyst rates between groups. Main results and the role of chance The manual control, Group A (n = 103), showed 99% survival rate and 92.2% blastocyst rate vs. 99% and 85.8% respectively, when performing the automatic protocol, Group B (n = 107). Chi square test was performed in order to compare survival and blastocyst rates between protocols. No signi