P-109 Nanoflakes of molybdenum disulfide functionalized with catechins modulate the sperm capacitation resulting in an improvement of the IVF outcomes in a swine in vitro model

Abstract Study question Are there any potential effects derived from the exposure of spermatozoa to MoS2/catechins nanoflakes during the process of capacitation? Summary answer The spermatozoa exposure to MoS2/catechin nanoflakes do not induce toxicity on spermatozoa and modulate sperm capacitation in a swine in vitro model. What is known already Molybdenum disulfide (MoS2)is a 2D graphene-like material widely used in a pletora of applications, such as energy storage, catalysts, sensors, lubricants and friction reducers. The unique features of this nanomaterial suggest interesting applications in the biomedical field. To date, there are no data regarding the potential effects of MoS2 nanoflakes on the reproductive field. Study design, size, duration To evaluate the potential effects derived from the interaction between the sperm cells and this material, spermatozoa were exposed to MoS2/Catechins nanoflakes at 10, 1, 0.1 ppm, using catechins as control, during capacitation. Different sperm capacitation events were studied, mainly related to their physic-chemical properties (membrane) and biochemical pathways (intracellular signaling), and then IVF experiments were performed with MoS2/catechins and catechins (0.1 ppm). Ten independent experiments were carried out between November 2020 and November 2021. Participants/materials, setting, methods The sonochemical exfoliation of bulk MoS2 in water-soluble MoS2 nanoflakes was obtained with a flavonoid, catechin (CT), that acts as stabilizing agent remaining anchored onto the surface of the MoS2 sheets. Sperm cells from different animals were included in this study. To evaluate the parameters exposed, different techniques were used: fluorescence microscopy, flow cytometry, western blot and in vitro fertilization assay. Main results and the role of chance No significant differences were found in terms of sperm membrane modifications (acrosome damage and membrane disorder) and sperm biochemical pathways (PKA activity, tyrosine phosphorylation patterns, intracellular calcium concentration and mitochondrial activity) between the groups of study compared to the control group. From the results arise that MoS2/CT nanoflakes do not induce any negative effect on the parameters evaluated related to sperm capacitation. Moreover, the addition of MoS2/CT nanoflakes and CT alone at a specific concentration (0.1 ppm) has demonstrated to increase the spermatozoa fertilizing ability in an IVF assay by increasing the number of fert