P-062 Morphokinetic embryo division timings and top-quality cleavage and blastocyst rates are unaffected by sperm quality

Abstract Study question Does testicular extracted sperm affect embryo morphokinetics and the rate of top-quality cleavage and blastocyst embryos. Summary answer Sperm origin, whether ejaculated normal sperm or testicular extracted sperm, was not found to affect embryo morphokinetic timings or top-quality embryo rates. What is known already Time-lapse monitoring (TLM) allows for continuous observation of the developing embryo detecting continuous embryo morphologic and kinetic changes from the zygote to the blastocyst stage. It eliminates potential unnecessary environmental fluctuations and diminishes inter/intra-observer variability compared to the traditional static morphologic evaluation of embryos requiring removal of the embryos from the incubator. Previous studies found sperm origin to affect embryo morphokinetics with some showing slower developmental rates with partially slower morphokinetic milestones with testicular sperm than embryos from ejaculated sperm. Study design, size, duration This retrospective monocentric study evaluated embryos derived from patients using ejaculated normal sperm compared to testicular extracted sperm. Embryo division timings and morphokinetic top-quality embryo rates were defined according to an in-house model for embryo selection based on known implantation data (KID) embryos. Top-quality embryos with the highest chance to implant were defined: tPNf