Assessment of of twenty-five variants of polymorphic genes for lipid and carbohydrate metabolism, vascular inflammation and the neurotransmitter system in the first non-cardioembolic ischemic stroke

Abstract The study of the relationship between various of gene polymorphism: APOA-V (rs34282181), APOA-V (rs619054), APOC-IV (rs1132899), LPL (rs199675233), LP(a) (rs41267817), APOB (rs1042031), APOB (rs676210), APOC-IV (rs1132899), APOH (rs4581), ANGPT4 (rs1044250), APOE (rs5174), APOE (rs 7412), APOE (rs 429358), APOD (rs7659), ADIPOQ (rs17366743), ADIPOQ (rs185847354), ADIROR (rs12342), TNFα (rs1800620), VEGFA (rs 62401172), IL6 (rs56383910), IL8 (rs1803205), MTHFR (rs1801131), MTHFR (rs1801133), GRM 1 (rs1047005), GRM 3 (rs2228595), BDNF (rs6265) and the development of the first non-cardioembolic ischemic stroke Material and methods The alleles frequencies and genotypes assessed for 25 mono-nucleotide polymorphic gene variants in 206 patients, who had first non-cardioembolic ischemic stroke, and 206 persons with no stroke, comparable with age, gender, place of living and ethnicity. Genotyping of polymorphisms was done with the prepared TaqMan probes. Genotyping of single nucleotide polymorphisms of genes: APOA-V (rs34282181), APOA-V (rs619054), APOC-IV (rs1132899), LPL (rs199675233), LP(a) (rs41267817), APOB (rs1042031), APOB (rs676210), APOC-IV (rs1132899), APOH (rs4581), ANGPT4 (rs1044250), APOE (rs5174), APOE (rs 7412), APOE (rs 429358), APOD (rs7659), ADIPOQ (rs17366743), ADIPOQ (rs185847354), ADIROR (rs12342), TNFα (rs1800620), VEGFA (rs 62401172), IL6 (rs56383910), IL8 (rs1803205), MTHFR (rs1801131), MTHFR (rs1801133), GRM 1 (rs1047005), GRM 3 (rs2228595), BDNF (rs6265) using ready-made TaqMan probes was carried out. Results For polymorphisms ApoD (rs7659), ApoA5 (rs619054), ApoB (rs676210), IL8 (rs1803205) there was significant difference between groups in the variety of minor alleles and genotypes. Conclusions There is significant relation of mononucleotide polymorhpisms of the genes APOD (rs7659), ApoA5 (rs619054), ApoB (rs676210), IL8 (rs1803205) with the development of the first non-cardioembolic ischemic stroke In the studied groups. Funding Acknowledgement Type of funding source: Public hospital(s). Main funding source(s): Moscow Center for Neurorehabilitation