P319 Genetic polymorphism of lactose intolerance in patients with Inflammatory Bowel Disease

Abstract Background Lactase deficiency is a disorder of lactose absorption in the body due to a deficiency of lactase, an enzyme that helps break down milk sugar. . The most common cause of primary lactase deficiency is due to a polymorphism in the MCM6 gene, which affects the function of the LCT lactase gene. Normal lactose digestibility is associated with the TT genotype, and when thymine is substituted for cytosine (allele C), a decrease in lactase levels in adulthood and impaired lactose digestibility to the point of complete inability to digest lactose should be expected. Understanding the prevalence of lactose intolerance in populations with inflammatory bowel disease may have important implications, preventing unnecessary restrictive diets and thereby helping to prevent future complications, including malnutrition and the occurrence of disorders of phosphorus-calcium metabolism. Aim Of The Study to investigate the prevalence of lactose intolerance and to evaluate the possibilities of correlation between genotype and lesion localization in IBD Methods Three single nucleotide polymorphisms of the LCT gene: LCT-13910 CC, LCT-13910 CT, LCT-13910 TT were analyzed in the group of patients with IBD (128 patients; UC-62 patients, CD 66 patients) and in the control group (45 patients with IBS). Results Baseline patient demographics and genetic analysis scores for lactase deficiency and variants of genetic polymorphism and association with localization of pathological process in IBD are presented in Table 1 and Figure 1. Conclusion Thus, the prevalence of LCT-13910 CC was 59.4% (congenital deficiency) and LCT-13910 CT was 31.3% (age-associated deficiency). In 9.4% of patients with IBD, no deficiency was genetically identified. In patients in the control group, LCT-13910 CC was detected in 15.5%, LCT-13910 CT in 42.4%, and LCT-13910 TT in 44.4%. LCT-13910 CC variant was predominant in CD patients with small intestinal lesions (n=41; 95.3% and 62.1%, respectively), in patients with left-sided ulcerative colitis (n=14; 51.8% and 22.6%, respectively) and total lesions in ulcerative colitis (n=12; 70.6% and 19.3%, respectively).Thus, the need for a lactose elimination diet is necessary in cases with small intestinal involvement in CD, and with total and left-sided course in UC.