B-205 A Simple Method for Opioids, Synthetic Opioids and PCP Detection in Hair Samples by LC-MS/MS

Abstract Background Hair analysis is useful for monitoring exposure to drugs and is a unique body material for the retrospective detection of drug consumption due to its large detection window, and it is easy to collect, store, and transport. Establishing a LC-MS/MS protocol for opioids determination may be challenging due to similarities among the molecular structures and the MS/MS fragmentation profile. This study aimed to develop and validate a simple, rapid, and sensitive LC-MS/MS method for quantification of 6-acetylcodeine, 6-acetylmorphine, codeine, dihydrocodeine, EDDP, PCP, fentanyl, heroin, hydrocodone, hydromorphone, meperidine, methadone, morphine, oxycodone, oxymorphone and tramadol in hair samples. Methods In this method, 15 mg of hair in a 2.0 mL microtube was decontaminated sequentially with dichloromethane and methanol. The sample was dried, and 0.75 mL of extraction solvent and a labeled internal standards solution were added. The hair sample was pulverized in a FastPrep-24™ 5G from MP-Biomedicals and then sonicated at 60°C for 60 min. The sample was centrifuged and filtered with Captiva filtration plates. Then, 100 µL of supernatant was added to 100 uL of ultrapure water and 5.0 uL was injected directly into the LC-MS/MS system. Chromatographic separation was performed on an Agilent 1290 Infinity II equipped with HALO Biphenyl column, and employing a mobile phase constituted by acetonitrile with 0.1% of formic acid and 5 mM of ammonium formate. The detection was performed with a Sciex 6500+ QTrap mass spectrometer. Results The current method was successfully validated for carryover, linearity, precision, recovery, limits of detection and quantification, and the measurement of uncertainty was calculated. The results were summarized in the Table 1. Conclusion The developed LC-MS/MS method was simple, rapid, and sensitive to detect opioids in hair samples. Table 1. Results of the validation parameters for determination of opioids and PCP in hair samples. Analytes Linearity LD LQ Precision Recovery Uncertainty 6-Acetylcodeine, 6-acetylmorphine, codeine, dihydrocodeine, PCP, fentanyl, heroin, hydrocodone, hydromorphone, meperidine, methadone, morphine, oxycodone, oxymorphone, tramadol 80–1000 pg/mg 0,08–10 pg/mg 80 pg/mg