Simultaneous determination of ethylene glycol and its major toxic metabolite, glycolic acid, in serum by gas chromatography

We developed a gas-chromatographic procedure for the simultaneous determination of ethylene glycol (EG) and its major toxic metabolite, glycolic acid (GA), suitable for clinical use in instances of EG intoxication. After serum protein precipitation with acetonitrile (containing internal standard), t...

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Veröffentlicht in:	Clinical chemistry (Baltimore, Md.) Md.), 1996-02, Vol.42 (2), p.292-297
Hauptverfasser:	Yao, HH, Porter, WH
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetonitriles Biological and medical sciences Capillary Action Chemical Precipitation Chromatography, Gas - methods Ethylene Glycol Ethylene Glycols - blood Ethylene Glycols - poisoning Food toxicology Glycolates - blood Humans Medical sciences Sensitivity and Specificity Toxicology
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Zusammenfassung:	We developed a gas-chromatographic procedure for the simultaneous determination of ethylene glycol (EG) and its major toxic metabolite, glycolic acid (GA), suitable for clinical use in instances of EG intoxication. After serum protein precipitation with acetonitrile (containing internal standard), the supernate is treated with 2,2-dimethoxypropane (containing dimethylformamide) to remove water, and the volume is then reduced by evaporation to
ISSN:	0009-9147 1530-8561
DOI:	10.1093/clinchem/42.2.292