Analysis and Quantitation of Rotenoids and Flavonoids in Derris (Lonchocarpus urucu) by High-Temperature High-Resolution Gas Chromatography

A glass capillary column coated with PS-086 (15% phenyl-80% methylpolysiloxane, 15 m × 0.30-mm i.d., 0.1-µm film thickness) is used to analyze extracts from Lonchocarpus urucu (Derris urucu). Several secondary metabolites (8 flavonoids, 10 rotenoids) are characterized without derivatization, and the...

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Veröffentlicht in:Journal of chromatographic science 2000-04, Vol.38 (4), p.174-180
Hauptverfasser: dos Santos Pereira, Alberto, Serrano, Maria Angelis Afonso, de Aquino Neto, Francisco Radler, da Cunha Pinto, Angelo, Texeira, Dulcineia Furtado, Gilbert, Benjamin
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Sprache:eng
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Zusammenfassung:A glass capillary column coated with PS-086 (15% phenyl-80% methylpolysiloxane, 15 m × 0.30-mm i.d., 0.1-µm film thickness) is used to analyze extracts from Lonchocarpus urucu (Derris urucu). Several secondary metabolites (8 flavonoids, 10 rotenoids) are characterized without derivatization, and the rotenoids are quantitated by high-temperature high-resolution gas chromatography (HTHRGC) and HTHRGC coupled with mass spectrometry (HTHRGC-MS). The limit of detection in flame ionization detection of rotenone is approximately 0.5 µg/mL, and the limit of quantitation was 2 µg/mL. Derris urucu bark is an excellent source of rotenone isomers (80 mg/g), deguelin (30 mg/g), and rotenolone (26 mg/g). Single solvent extractions (hexane, methylene dichloride, acetone, or methanol) are not able to fully extract the flavonoids and rotenoids. Complete extraction is achieved using a mixture of methanol-methylene dichloride (1:1), indicating a complex association of these compounds with the plant tissue. HTHRGC and HTHRGC-MS are shown to be quick and informative tools for the rapid analysis of crude extracts without the need for prior derivatization and fractionation.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/38.4.174