High-Performance Thin-Layer Chromatographic Analysis of Thiazine Dyes on Silica, Cyano, and Reversed-Phase C18 Layers

The high-performance thin-layer chromatographic (HPTLC) analysis of thiazine dyes is performed on silica, cyano, and reversed-phase layers. We examine different parameters (organic modifier, relative humidity, temperature, preloading, and pH) influencing the chromatography of these quaternary ammonium compounds. We eventually select the analysis on silica as it is superior in terms of resolution and band form. The separation power of the method is equivalent to that of high-performance liquid chromatography (HPLC) but appears superior in terms of speed and sample turnover.