Resistance of A-431 epidermoid carcinoma cells to early membrane changes induced by tumour promoters

The A-431 human epidermoid carcinoma cell line was resistant to 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibition of epidermal growth factor binding, enhanced incorporation of [3H]choline into phospholipids and uptake of 86Rb and [3H]2-deoxyglucose. The cells were also resistant to TPA-stimulate...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Carcinogenesis (New York) 1982, Vol.3 (7), p.837-839
Hauptverfasser:	Froscio, Mario, Tapley, Peter M., Guy, Graeme R., Pappalardo, Sylvia, Jones, Meril J., Murray, Andrew W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Arachidonic Acid Arachidonic Acids - metabolism Carcinogens - pharmacology Carcinoma, Squamous Cell - metabolism Cells, Cultured Choline - metabolism Drug Resistance Humans Membranes - drug effects Membranes - metabolism Neoplasms, Experimental - metabolism Phospholipids - metabolism Tetradecanoylphorbol Acetate - pharmacology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The A-431 human epidermoid carcinoma cell line was resistant to 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibition of epidermal growth factor binding, enhanced incorporation of [3H]choline into phospholipids and uptake of 86Rb and [3H]2-deoxyglucose. The cells were also resistant to TPA-stimulated release of radioactive choline derivatives and aracholdonic acid from cells prelabelled with [3H]choline or [14C]arachidonic acid, respectively. The A-431 cells did not metabolise [3H]TPA. Despite their TPA-unresponsiveness, A-431 cells contained specific, high affinity binding sites for [3H]phorbol-12,13-dibutyrate with characteristics similar to other cultured cell lines.
ISSN:	0143-3334 1460-2180
DOI:	10.1093/carcin/3.7.837