Impaired restriction endonuclease cleavage of DNA modified with N-methyl-N-nitrosourea

Impaired restriction endonuclease cleavage of DNA modified with N-methyl-N-nitrosourea

This paper examines the ability of several restriction enzymes to cleave lambda DNA treated in vitro with N-methyl-N-nitrosourea. An impaired digestion was observed with all tested enzymes suggesting that the sequence specific restriction endonucleases are either unable to recognize carcinogen-modif...

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Veröffentlicht in:Carcinogenesis (New York) 1980-08, Vol.1 (8), p.729-731
Hauptverfasser: Boehm, Thomas L.J., Drahovsky, Dusan
Format: Artikel
Sprache:eng
Schlagworte:
Bacteriophage lambda - genetics
Carcinogens - pharmacology
DNA Adducts - chemistry
DNA Damage
DNA Restriction Enzymes - pharmacology
DNA, Viral - drug effects
Electrophoresis, Agar Gel
Methylnitrosourea - pharmacology
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Zusammenfassung:This paper examines the ability of several restriction enzymes to cleave lambda DNA treated in vitro with N-methyl-N-nitrosourea. An impaired digestion was observed with all tested enzymes suggesting that the sequence specific restriction endonucleases are either unable to recognize carcinogen-modified sequences or to insert endonucleolytic cuts at such sites.
ISSN:0143-3334
1460-2180
DOI:10.1093/carcin/1.8.729