The Regulatory Effect of VEGF-Ax on Rat Bone Marrow Mesenchymal Stem Cells' Angioblastic Differentiation and Its Proangiogenic Ability
Vascular endothelial growth factor A (VEGFA), which plays a key role in angiogenesis, is composed of many isoforms. Distinct VEGFA isoforms are generated by alternative splicing of VEGFA mRNA and named as VEGF xxx , where xxx represents the number of amino acids present in the final protein sequence...
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| Veröffentlicht in: | Stem cells and development 2020-05, Vol.29 (10), p.667-677 |
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| creator | Li, Jianjun Li, Zhihao Wang, Chengqiang Li, Zhijia Xu, Haixia Hu, Yunteng Tan, Zhiwen Zhang, Fu Liu, Chun Yang, Minsheng Wang, Yihan Jin, Yanglei Peng, Ziyue Biswas, Sourabh Zhu, Lixin |
| description | Vascular endothelial growth factor A (VEGFA), which plays a key role in angiogenesis, is composed of many isoforms. Distinct VEGFA isoforms are generated by alternative splicing of VEGFA mRNA and named as VEGF
xxx
, where
xxx
represents the number of amino acids present in the final protein sequence. These isoforms have opponent pro- and antiangiogenic effects. VEGF-Ax, an additional isoform containing a 22-amino-acid extension in the COOH terminus, arising from VEGFA mRNA, programmed translational readthrough. The function of VEGF-Ax is not clear, especially the conclusion that VEGF-Ax regulates angiogenesis is contradictory. Thus, we investigated the effect of VEGF-Ax on differentiation and angiogenesis of rat bone marrow mesenchymal stem cells (BMMSCs). The results showed that VEGF-Ax could promote the proliferation and migration of BMMSCs, stimulate the differentiation of BMMSCs into endothelial cell-like cells, and protect BMMSCs from endoplasmic reticulum stress-induced apoptosis. |
| doi_str_mv | 10.1089/scd.2019.0198 |
| format | Article |
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xxx
, where
xxx
represents the number of amino acids present in the final protein sequence. These isoforms have opponent pro- and antiangiogenic effects. VEGF-Ax, an additional isoform containing a 22-amino-acid extension in the COOH terminus, arising from VEGFA mRNA, programmed translational readthrough. The function of VEGF-Ax is not clear, especially the conclusion that VEGF-Ax regulates angiogenesis is contradictory. Thus, we investigated the effect of VEGF-Ax on differentiation and angiogenesis of rat bone marrow mesenchymal stem cells (BMMSCs). The results showed that VEGF-Ax could promote the proliferation and migration of BMMSCs, stimulate the differentiation of BMMSCs into endothelial cell-like cells, and protect BMMSCs from endoplasmic reticulum stress-induced apoptosis.</description><identifier>ISSN: 1547-3287</identifier><identifier>EISSN: 1557-8534</identifier><identifier>DOI: 10.1089/scd.2019.0198</identifier><identifier>PMID: 32079499</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>Alternative Splicing - drug effects ; Angiogenesis Inhibitors - pharmacology ; Animals ; Bone Marrow Cells - drug effects ; Bone Marrow Cells - metabolism ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; Endothelial Cells - drug effects ; Endothelial Cells - metabolism ; Mesenchymal Stem Cells - drug effects ; Mesenchymal Stem Cells - metabolism ; Neovascularization, Physiologic - drug effects ; Neovascularization, Physiologic - physiology ; Original Research Reports ; Rats, Sprague-Dawley ; Vascular Endothelial Growth Factor A - metabolism</subject><ispartof>Stem cells and development, 2020-05, Vol.29 (10), p.667-677</ispartof><rights>2020, Mary Ann Liebert, Inc., publishers</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-8ed61449562e9f50a54baea8d4f09d6d5507fff5959dcbe80b5ad9be5dfe09903</citedby><cites>FETCH-LOGICAL-c337t-8ed61449562e9f50a54baea8d4f09d6d5507fff5959dcbe80b5ad9be5dfe09903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32079499$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Jianjun</creatorcontrib><creatorcontrib>Li, Zhihao</creatorcontrib><creatorcontrib>Wang, Chengqiang</creatorcontrib><creatorcontrib>Li, Zhijia</creatorcontrib><creatorcontrib>Xu, Haixia</creatorcontrib><creatorcontrib>Hu, Yunteng</creatorcontrib><creatorcontrib>Tan, Zhiwen</creatorcontrib><creatorcontrib>Zhang, Fu</creatorcontrib><creatorcontrib>Liu, Chun</creatorcontrib><creatorcontrib>Yang, Minsheng</creatorcontrib><creatorcontrib>Wang, Yihan</creatorcontrib><creatorcontrib>Jin, Yanglei</creatorcontrib><creatorcontrib>Peng, Ziyue</creatorcontrib><creatorcontrib>Biswas, Sourabh</creatorcontrib><creatorcontrib>Zhu, Lixin</creatorcontrib><title>The Regulatory Effect of VEGF-Ax on Rat Bone Marrow Mesenchymal Stem Cells' Angioblastic Differentiation and Its Proangiogenic Ability</title><title>Stem cells and development</title><addtitle>Stem Cells Dev</addtitle><description>Vascular endothelial growth factor A (VEGFA), which plays a key role in angiogenesis, is composed of many isoforms. Distinct VEGFA isoforms are generated by alternative splicing of VEGFA mRNA and named as VEGF
xxx
, where
xxx
represents the number of amino acids present in the final protein sequence. These isoforms have opponent pro- and antiangiogenic effects. VEGF-Ax, an additional isoform containing a 22-amino-acid extension in the COOH terminus, arising from VEGFA mRNA, programmed translational readthrough. The function of VEGF-Ax is not clear, especially the conclusion that VEGF-Ax regulates angiogenesis is contradictory. Thus, we investigated the effect of VEGF-Ax on differentiation and angiogenesis of rat bone marrow mesenchymal stem cells (BMMSCs). The results showed that VEGF-Ax could promote the proliferation and migration of BMMSCs, stimulate the differentiation of BMMSCs into endothelial cell-like cells, and protect BMMSCs from endoplasmic reticulum stress-induced apoptosis.</description><subject>Alternative Splicing - drug effects</subject><subject>Angiogenesis Inhibitors - pharmacology</subject><subject>Animals</subject><subject>Bone Marrow Cells - drug effects</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>Endothelial Cells - drug effects</subject><subject>Endothelial Cells - metabolism</subject><subject>Mesenchymal Stem Cells - drug effects</subject><subject>Mesenchymal Stem Cells - metabolism</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Neovascularization, Physiologic - physiology</subject><subject>Original Research Reports</subject><subject>Rats, Sprague-Dawley</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><issn>1547-3287</issn><issn>1557-8534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQQC0EoqUwsiJvTCnOh5N4DKUtlVqBSmGNnPjcGiVOZbuC_AF-N4kKrAynO52e3vAQuvbJ2Ccpu7OlGAfEZ-Nu0hM09ClNvJSG0Wl_R4kXBmkyQBfWvhMSxEEanaNBGJCERYwN0ddmB3gN20PFXWNaPJUSSocbid-m85mXfeJG4zV3-L7RgFfcmOYDr8CCLndtzSv84qDGE6gqe4szvVVNUXHrVIkfVKcyoJ3iTnUSrgVeOIufTcN7bgu6o7JCVcq1l-hM8srC1c8eodfZdDN59JZP88UkW3plGCbOS0HEfhQxGgfAJCWcRgUHnopIEiZiQSlJpJSUUSbKAlJSUC5YAVRIIIyRcIS8o7c0jbUGZL43quamzX2S9z3zrmfe98z7nh1_c-T3h6IG8Uf_BuyA8Aj0b651paAA4_7RfgMQXIQg</recordid><startdate>20200515</startdate><enddate>20200515</enddate><creator>Li, Jianjun</creator><creator>Li, Zhihao</creator><creator>Wang, Chengqiang</creator><creator>Li, Zhijia</creator><creator>Xu, Haixia</creator><creator>Hu, Yunteng</creator><creator>Tan, Zhiwen</creator><creator>Zhang, Fu</creator><creator>Liu, Chun</creator><creator>Yang, Minsheng</creator><creator>Wang, Yihan</creator><creator>Jin, Yanglei</creator><creator>Peng, Ziyue</creator><creator>Biswas, Sourabh</creator><creator>Zhu, Lixin</creator><general>Mary Ann Liebert, Inc., publishers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20200515</creationdate><title>The Regulatory Effect of VEGF-Ax on Rat Bone Marrow Mesenchymal Stem Cells' Angioblastic Differentiation and Its Proangiogenic Ability</title><author>Li, Jianjun ; Li, Zhihao ; Wang, Chengqiang ; Li, Zhijia ; Xu, Haixia ; Hu, Yunteng ; Tan, Zhiwen ; Zhang, Fu ; Liu, Chun ; Yang, Minsheng ; Wang, Yihan ; Jin, Yanglei ; Peng, Ziyue ; Biswas, Sourabh ; Zhu, Lixin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-8ed61449562e9f50a54baea8d4f09d6d5507fff5959dcbe80b5ad9be5dfe09903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Alternative Splicing - drug effects</topic><topic>Angiogenesis Inhibitors - pharmacology</topic><topic>Animals</topic><topic>Bone Marrow Cells - drug effects</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>Endothelial Cells - drug effects</topic><topic>Endothelial Cells - metabolism</topic><topic>Mesenchymal Stem Cells - drug effects</topic><topic>Mesenchymal Stem Cells - metabolism</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Neovascularization, Physiologic - physiology</topic><topic>Original Research Reports</topic><topic>Rats, Sprague-Dawley</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Jianjun</creatorcontrib><creatorcontrib>Li, Zhihao</creatorcontrib><creatorcontrib>Wang, Chengqiang</creatorcontrib><creatorcontrib>Li, Zhijia</creatorcontrib><creatorcontrib>Xu, Haixia</creatorcontrib><creatorcontrib>Hu, Yunteng</creatorcontrib><creatorcontrib>Tan, Zhiwen</creatorcontrib><creatorcontrib>Zhang, Fu</creatorcontrib><creatorcontrib>Liu, Chun</creatorcontrib><creatorcontrib>Yang, Minsheng</creatorcontrib><creatorcontrib>Wang, Yihan</creatorcontrib><creatorcontrib>Jin, Yanglei</creatorcontrib><creatorcontrib>Peng, Ziyue</creatorcontrib><creatorcontrib>Biswas, Sourabh</creatorcontrib><creatorcontrib>Zhu, Lixin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Stem cells and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jianjun</au><au>Li, Zhihao</au><au>Wang, Chengqiang</au><au>Li, Zhijia</au><au>Xu, Haixia</au><au>Hu, Yunteng</au><au>Tan, Zhiwen</au><au>Zhang, Fu</au><au>Liu, Chun</au><au>Yang, Minsheng</au><au>Wang, Yihan</au><au>Jin, Yanglei</au><au>Peng, Ziyue</au><au>Biswas, Sourabh</au><au>Zhu, Lixin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Regulatory Effect of VEGF-Ax on Rat Bone Marrow Mesenchymal Stem Cells' Angioblastic Differentiation and Its Proangiogenic Ability</atitle><jtitle>Stem cells and development</jtitle><addtitle>Stem Cells Dev</addtitle><date>2020-05-15</date><risdate>2020</risdate><volume>29</volume><issue>10</issue><spage>667</spage><epage>677</epage><pages>667-677</pages><issn>1547-3287</issn><eissn>1557-8534</eissn><abstract>Vascular endothelial growth factor A (VEGFA), which plays a key role in angiogenesis, is composed of many isoforms. Distinct VEGFA isoforms are generated by alternative splicing of VEGFA mRNA and named as VEGF
xxx
, where
xxx
represents the number of amino acids present in the final protein sequence. These isoforms have opponent pro- and antiangiogenic effects. VEGF-Ax, an additional isoform containing a 22-amino-acid extension in the COOH terminus, arising from VEGFA mRNA, programmed translational readthrough. The function of VEGF-Ax is not clear, especially the conclusion that VEGF-Ax regulates angiogenesis is contradictory. Thus, we investigated the effect of VEGF-Ax on differentiation and angiogenesis of rat bone marrow mesenchymal stem cells (BMMSCs). The results showed that VEGF-Ax could promote the proliferation and migration of BMMSCs, stimulate the differentiation of BMMSCs into endothelial cell-like cells, and protect BMMSCs from endoplasmic reticulum stress-induced apoptosis.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>32079499</pmid><doi>10.1089/scd.2019.0198</doi><tpages>11</tpages></addata></record> |
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| ispartof | Stem cells and development, 2020-05, Vol.29 (10), p.667-677 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Alternative Splicing - drug effects Angiogenesis Inhibitors - pharmacology Animals Bone Marrow Cells - drug effects Bone Marrow Cells - metabolism Cell Differentiation - drug effects Cell Differentiation - physiology Endothelial Cells - drug effects Endothelial Cells - metabolism Mesenchymal Stem Cells - drug effects Mesenchymal Stem Cells - metabolism Neovascularization, Physiologic - drug effects Neovascularization, Physiologic - physiology Original Research Reports Rats, Sprague-Dawley Vascular Endothelial Growth Factor A - metabolism |
| title | The Regulatory Effect of VEGF-Ax on Rat Bone Marrow Mesenchymal Stem Cells' Angioblastic Differentiation and Its Proangiogenic Ability |
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