The Regulatory Effect of VEGF-Ax on Rat Bone Marrow Mesenchymal Stem Cells' Angioblastic Differentiation and Its Proangiogenic Ability

Vascular endothelial growth factor A (VEGFA), which plays a key role in angiogenesis, is composed of many isoforms. Distinct VEGFA isoforms are generated by alternative splicing of VEGFA mRNA and named as VEGF xxx , where xxx represents the number of amino acids present in the final protein sequence...

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Veröffentlicht in:Stem cells and development 2020-05, Vol.29 (10), p.667-677
Hauptverfasser: Li, Jianjun, Li, Zhihao, Wang, Chengqiang, Li, Zhijia, Xu, Haixia, Hu, Yunteng, Tan, Zhiwen, Zhang, Fu, Liu, Chun, Yang, Minsheng, Wang, Yihan, Jin, Yanglei, Peng, Ziyue, Biswas, Sourabh, Zhu, Lixin
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Sprache:eng
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Zusammenfassung:Vascular endothelial growth factor A (VEGFA), which plays a key role in angiogenesis, is composed of many isoforms. Distinct VEGFA isoforms are generated by alternative splicing of VEGFA mRNA and named as VEGF xxx , where xxx represents the number of amino acids present in the final protein sequence. These isoforms have opponent pro- and antiangiogenic effects. VEGF-Ax, an additional isoform containing a 22-amino-acid extension in the COOH terminus, arising from VEGFA mRNA, programmed translational readthrough. The function of VEGF-Ax is not clear, especially the conclusion that VEGF-Ax regulates angiogenesis is contradictory. Thus, we investigated the effect of VEGF-Ax on differentiation and angiogenesis of rat bone marrow mesenchymal stem cells (BMMSCs). The results showed that VEGF-Ax could promote the proliferation and migration of BMMSCs, stimulate the differentiation of BMMSCs into endothelial cell-like cells, and protect BMMSCs from endoplasmic reticulum stress-induced apoptosis.
ISSN:1547-3287
1557-8534
DOI:10.1089/scd.2019.0198