ADAM33 Gene Polymorphisms Are Not Associated with Asthma in Turkish Children

A disintegrin and metalloprotease domain 33 ( ADAM33 ) is the first asthma-susceptible gene discovered by positional cloning. The objective of this study was to determine the association between ADAM33 polymorphisms and asthma in the Turkish children population with asthma. Four single-nucleotide polymorphisms (SNPs) previously reported to be related with asthma were genotyped in 98 cases and 100 controls. The genotyping procedure consisted of a polymerase chain reaction amplification and SNP detection of the 12433 T/C (T1), 12462 C/T (T2), 12540 C/T (T+1), and 12601 T/G (T+2) variants of the ADAM33 gene. No significant differences were observed for T1, T2, T+1, and T+2 polymorphisms between asthmatic patients and controls. There was also no difference between the atopic and nonatopic asthma group by means of ADAM33 polymorphisms. Our data revealed that there is no association of these 4 SNPs of the ADAM33 gene with asthma in the Turkish children population.