Biomineralization guided by polydopamine-modifed poly(L-lactide) fibrous membrane for promoted osteoconductive activity

A method to mediate biomineralization of electrospinning poly(L-lactide) (PLLA) fibrous membrane assisted by polydopamine (PDA) coating was developed to obtain enhanced osteoconductive activity. The biomineralization mechanism, surface composition, morphology and hydrophilicity of the original and modified PLLA fibrous membranes were characterized. Results revealed that the PDA coating effectively accelerated the formation of hydroxyapatite (HA) on PLLA fibrous membrane and resulted a great increase in hydrophilicity. Moreover, the tensile property of PLLA fibrous membrane was enhanced by the PDA coating while almost kept unchanged by further immobilized with HA. Cells culture results indicated that the successive introduction of PDA and HA contributed to an obvious improvement in the adhesion and proliferation, as well as up-regulated alkaline phosphatase (ALP) activity and promoted osteogenic-related genes and proteins expression of MC3T3-E1 cells. Overall, the as-prepared PLLA-PDA-HA fibrous membrane can be expected as a favorable scaffold for bone tissue repair.