Sensitive visual detection of intracellular zinc ions based on signal-on polydopamine carbon dots

The concentration of intracellular zinc ions is a significant clinical parameter for diagnosis. However, it is still a challenge for direct visual detection of zinc ions in cells at single-cell level. To address this issue, herein, water-soluble amino-rich polydopamine carbon quantum dots (PDA-CQDs)...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nanotechnology 2022-04, Vol.33 (18), p.185502
Hauptverfasser: Ni, Jiatong, Kong, Lixiang, Tang, Minglu, Song, Yan, Zhao, Junge, Wang, Wenxin, Sun, Tiedong, Wang, Ying, Wang, Lei
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The concentration of intracellular zinc ions is a significant clinical parameter for diagnosis. However, it is still a challenge for direct visual detection of zinc ions in cells at single-cell level. To address this issue, herein, water-soluble amino-rich polydopamine carbon quantum dots (PDA-CQDs) were successfully synthesized, with strong blue-green fluorescence as the probes for zinc ions detection in cells. The structure and properties of PDA-CQDs were confirmed by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transformed infrared (FT-IR), UV-visible spectrophotometry (UV-vis), and fluorescence spectroscopy. Importantly, by successfully linking salicylaldehyde (SA) to PDA-CQDs via nucleophilic reaction, the FL quenching and Zn ions induced FL-recovering system was built up, thus offering a signal-on platform for the detection of zinc ions. This PDA-CQDs-SA nanoprobe can be applied for the detection of Zn with a detection limit of 0.09 M, with good biocompatibility confirmed using cytotoxicity assay. Of significance, the results of fluorescence bioimaging showed that PDA-CQDs-SA is able to detect Zn in single-cell visually, with the detection limit of Zn ions in cells as low as 0.11 M per cell, which was confirmed using flow cytometry. Therefore, this work offers a potential probe for Zn detection in cells at single-cell level, towards the precise diagnosis of zinc ions related diseases.
ISSN:0957-4484
1361-6528
DOI:10.1088/1361-6528/ac4dc1