SEPARATION OF THE BRYOSTATIN DERIVATIVES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

SEPARATION OF THE BRYOSTATIN DERIVATIVES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

The Separation of bryostatin derivatives (26-acetate, 26-metabromobenzoate, 26-ketone, and 13→ 30 epoxide) by high performance liquid chromatography (HPLC) using normal phase and reverse phase systems was accomplished. Using n-hexane/EtOAc/MeOH/H 2 O (26:5:1:0.01) as mobile phase for normal phase HP...

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Veröffentlicht in:Journal of liquid chromatography & related technologies 2000-01, Vol.23 (3), p.399-409
Hauptverfasser: Kamano, Yoshiaki, Kotake, Ayano, Nogawa, Toshihiko, Hiraide, Hatsue, Pettit, George R., Herald, Cherry L.
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
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Zusammenfassung:The Separation of bryostatin derivatives (26-acetate, 26-metabromobenzoate, 26-ketone, and 13→ 30 epoxide) by high performance liquid chromatography (HPLC) using normal phase and reverse phase systems was accomplished. Using n-hexane/EtOAc/MeOH/H 2 O (26:5:1:0.01) as mobile phase for normal phase HPLC and acetonitrile (CH 3 CN)/H 2 O system for reverse phase HPLC provided good separation of bryostatin derivatives. In addition, contributions of functional group for eluate order were discussed.
ISSN:1082-6076
1520-572X
DOI:10.1081/JLC-100101459