The effect of early probiotic exposure on the preterm infant gut microbiome development
Premature birth, especially if born before week 32 of gestation, is associated with increased risk of neonatal morbidity and mortality. Prophylactic use of probiotics has been suggested to protect preterm infants via supporting a healthy gut microbiota (GM) development, but the suggested strains and...
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| Veröffentlicht in: | Gut microbes 2021-01, Vol.13 (1), p.1951113-1951113 |
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| creator | Hui, Yan Smith, Birgitte Mortensen, Martin Steen Krych, Lukasz Sørensen, Søren J. Greisen, Gorm Krogfelt, Karen Angeliki Nielsen, Dennis Sandris |
| description | Premature birth, especially if born before week 32 of gestation, is associated with increased risk of neonatal morbidity and mortality. Prophylactic use of probiotics has been suggested to protect preterm infants via supporting a healthy gut microbiota (GM) development, but the suggested strains and doses vary between studies. In this study, we profiled the GM of 5, 10 and 30-day fecal samples from two cohorts of preterm neonates (born |
| doi_str_mv | 10.1080/19490976.2021.1951113 |
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Abbreviations: GM: Gut microbiota; ASV: Amplicon sequence variant; NEC: Necrotizing enterocolitis; DOL: Days of life; NICU: Neonatal intensive care unit; ESPGHAN: European Society for Pediatric Gastroenterology, Hepatology and Nutrition; Db-RDA: Distance-based redundancy analysis; PERMANOVA: Permutational multivariate analysis of variance; ANCOM: Analysis of compositions of microbiomes; LGG: Lacticaseibacillus (former Lactobacillus) rhamnosus GG; BB-12: Bifidobacterium animalis ssp. lactis BB-12; DGGE: Denaturing Gradient Gel Electrophoresis</description><identifier>ISSN: 1949-0976</identifier><identifier>EISSN: 1949-0984</identifier><identifier>DOI: 10.1080/19490976.2021.1951113</identifier><identifier>PMID: 34264803</identifier><language>eng</language><publisher>United States: Taylor & Francis</publisher><subject>amplicon sequencing ; Bifidobacterium animalis - isolation & purification ; Cohort Studies ; Denmark ; Enterocolitis, Necrotizing - drug therapy ; Enterocolitis, Necrotizing - immunology ; Feces - microbiology ; Female ; Gastrointestinal Microbiome - drug effects ; Gastrointestinal Microbiome - immunology ; gut microbiome ; Humans ; Infant, Newborn ; Infant, Newborn, Diseases - drug therapy ; Infant, Newborn, Diseases - immunology ; Infant, Premature - growth & development ; Infant, Premature - immunology ; Lactobacillus - isolation & purification ; Male ; necrotizing enterocolitis ; Preterm ; probiotics ; Probiotics - pharmacology ; Probiotics - therapeutic use ; Research Paper</subject><ispartof>Gut microbes, 2021-01, Vol.13 (1), p.1951113-1951113</ispartof><rights>2021 The Author(s). Published with license by Taylor & Francis Group, LLC. 2021</rights><rights>2021 The Author(s). Published with license by Taylor & Francis Group, LLC. 2021 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c534t-38ebb6c4b60edab31e0fe0ab3b79ad6e18bfa0e6277ffdd46bf7e2bd95c868f63</citedby><cites>FETCH-LOGICAL-c534t-38ebb6c4b60edab31e0fe0ab3b79ad6e18bfa0e6277ffdd46bf7e2bd95c868f63</cites><orcidid>0000-0001-7536-3453 ; 0000-0001-6227-9906</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284123/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284123/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,27483,27905,27906,53772,53774,59122,59123</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34264803$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hui, Yan</creatorcontrib><creatorcontrib>Smith, Birgitte</creatorcontrib><creatorcontrib>Mortensen, Martin Steen</creatorcontrib><creatorcontrib>Krych, Lukasz</creatorcontrib><creatorcontrib>Sørensen, Søren J.</creatorcontrib><creatorcontrib>Greisen, Gorm</creatorcontrib><creatorcontrib>Krogfelt, Karen Angeliki</creatorcontrib><creatorcontrib>Nielsen, Dennis Sandris</creatorcontrib><title>The effect of early probiotic exposure on the preterm infant gut microbiome development</title><title>Gut microbes</title><addtitle>Gut Microbes</addtitle><description>Premature birth, especially if born before week 32 of gestation, is associated with increased risk of neonatal morbidity and mortality. Prophylactic use of probiotics has been suggested to protect preterm infants via supporting a healthy gut microbiota (GM) development, but the suggested strains and doses vary between studies. In this study, we profiled the GM of 5, 10 and 30-day fecal samples from two cohorts of preterm neonates (born <30 weeks of gestation) recruited in the same neonatal intensive care unit. One cohort (n = 165) was recruited from September 2006 to January 2009 before probiotics were introduced in the clinic. The second cohort (n = 87) was recruited from May 2010 to October 2011 after introducing Lacticaseibacillus rhamnosus GG and Bifidobacterium animalis ssp. lactis BB-12 supplementation policy. Through V3-V4 region 16S rRNA gene amplicon sequencing, a distinct increase of L. rhamnosus and B. animalis was found in the fecal samples of neonates supplemented with probiotics. During the first 30 days of life, the preterm GM went through similarly patterned progression of bacterial populations. Staphylococcus and Weissella dominated in early samples, but was gradually overtaken by Veillonella, Enterococcus and Enterobacteriaceae. Probiotic supplementation was associated with pronounced reduction of Weissella, Veillonella spp. and the opportunistic pathogen Klebsiella. Potential nosocomial pathogens Citrobacter and Chryseobacterium species also gradually phased out. In conclusion, probiotic supplementation to preterm neonates affected gut colonization by certain bacteria, but did not change the overall longitudinal bacterial progression in the neonatal period.
Abbreviations: GM: Gut microbiota; ASV: Amplicon sequence variant; NEC: Necrotizing enterocolitis; DOL: Days of life; NICU: Neonatal intensive care unit; ESPGHAN: European Society for Pediatric Gastroenterology, Hepatology and Nutrition; Db-RDA: Distance-based redundancy analysis; PERMANOVA: Permutational multivariate analysis of variance; ANCOM: Analysis of compositions of microbiomes; LGG: Lacticaseibacillus (former Lactobacillus) rhamnosus GG; BB-12: Bifidobacterium animalis ssp. lactis BB-12; DGGE: Denaturing Gradient Gel Electrophoresis</description><subject>amplicon sequencing</subject><subject>Bifidobacterium animalis - isolation & purification</subject><subject>Cohort Studies</subject><subject>Denmark</subject><subject>Enterocolitis, Necrotizing - drug therapy</subject><subject>Enterocolitis, Necrotizing - immunology</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Gastrointestinal Microbiome - drug effects</subject><subject>Gastrointestinal Microbiome - immunology</subject><subject>gut microbiome</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Infant, Newborn, Diseases - drug therapy</subject><subject>Infant, Newborn, Diseases - immunology</subject><subject>Infant, Premature - growth & development</subject><subject>Infant, Premature - immunology</subject><subject>Lactobacillus - isolation & purification</subject><subject>Male</subject><subject>necrotizing enterocolitis</subject><subject>Preterm</subject><subject>probiotics</subject><subject>Probiotics - pharmacology</subject><subject>Probiotics - therapeutic use</subject><subject>Research Paper</subject><issn>1949-0976</issn><issn>1949-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNp9kU1v1DAQhi0EotXSnwDykcsu_orjXBCo4qNSJS5FHC1_jLepkjjYTsv-e7zd7Ype8MWj8TvPeOZF6C0lG0oU-UA70ZGulRtGGN3QrqGU8hfofJ9fk06Jl6e4lWfoIuc7Uo8QLZH8NTrjgkmhCD9Hv25uAUMI4AqOAYNJww7PKdo-lt5h-DPHvCTAccKlKucEBdKI-ymYqeDtUvDYu0f5CNjDPQxxHmEqb9CrYIYMF8d7hX5-_XJz-X19_ePb1eXn67VruChrrsBa6YSVBLyxnAIJQGpg2854CVTZYAhI1rYheC-kDS0w67vGKamC5Ct0deD6aO70nPrRpJ2OptePiZi22qQ6yQCagFBeEWIMJ8JQrpysW-SEcCl5y11lfTyw5sWO4F0dI5nhGfT5y9Tf6m2814opQRmvgPdHQIq_F8hFj312MAxmgrhkzZqGdR2TVbtCzUFal5dzgnBqQ4nee6yfPNZ7j_XR41r37t8_nqqeHK2CTwdBtSim0TzENHhdzG6IKSQzuT5r_v8efwF9AbfJ</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Hui, Yan</creator><creator>Smith, Birgitte</creator><creator>Mortensen, Martin Steen</creator><creator>Krych, Lukasz</creator><creator>Sørensen, Søren J.</creator><creator>Greisen, Gorm</creator><creator>Krogfelt, Karen Angeliki</creator><creator>Nielsen, Dennis Sandris</creator><general>Taylor & Francis</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-7536-3453</orcidid><orcidid>https://orcid.org/0000-0001-6227-9906</orcidid></search><sort><creationdate>20210101</creationdate><title>The effect of early probiotic exposure on the preterm infant gut microbiome development</title><author>Hui, Yan ; Smith, Birgitte ; Mortensen, Martin Steen ; Krych, Lukasz ; Sørensen, Søren J. ; Greisen, Gorm ; Krogfelt, Karen Angeliki ; Nielsen, Dennis Sandris</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-38ebb6c4b60edab31e0fe0ab3b79ad6e18bfa0e6277ffdd46bf7e2bd95c868f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>amplicon sequencing</topic><topic>Bifidobacterium animalis - isolation & purification</topic><topic>Cohort Studies</topic><topic>Denmark</topic><topic>Enterocolitis, Necrotizing - drug therapy</topic><topic>Enterocolitis, Necrotizing - immunology</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Gastrointestinal Microbiome - drug effects</topic><topic>Gastrointestinal Microbiome - immunology</topic><topic>gut microbiome</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Infant, Newborn, Diseases - drug therapy</topic><topic>Infant, Newborn, Diseases - immunology</topic><topic>Infant, Premature - growth & development</topic><topic>Infant, Premature - immunology</topic><topic>Lactobacillus - isolation & purification</topic><topic>Male</topic><topic>necrotizing enterocolitis</topic><topic>Preterm</topic><topic>probiotics</topic><topic>Probiotics - pharmacology</topic><topic>Probiotics - therapeutic use</topic><topic>Research Paper</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hui, Yan</creatorcontrib><creatorcontrib>Smith, Birgitte</creatorcontrib><creatorcontrib>Mortensen, Martin Steen</creatorcontrib><creatorcontrib>Krych, Lukasz</creatorcontrib><creatorcontrib>Sørensen, Søren J.</creatorcontrib><creatorcontrib>Greisen, Gorm</creatorcontrib><creatorcontrib>Krogfelt, Karen Angeliki</creatorcontrib><creatorcontrib>Nielsen, Dennis Sandris</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Gut microbes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hui, Yan</au><au>Smith, Birgitte</au><au>Mortensen, Martin Steen</au><au>Krych, Lukasz</au><au>Sørensen, Søren J.</au><au>Greisen, Gorm</au><au>Krogfelt, Karen Angeliki</au><au>Nielsen, Dennis Sandris</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of early probiotic exposure on the preterm infant gut microbiome development</atitle><jtitle>Gut microbes</jtitle><addtitle>Gut Microbes</addtitle><date>2021-01-01</date><risdate>2021</risdate><volume>13</volume><issue>1</issue><spage>1951113</spage><epage>1951113</epage><pages>1951113-1951113</pages><issn>1949-0976</issn><eissn>1949-0984</eissn><abstract>Premature birth, especially if born before week 32 of gestation, is associated with increased risk of neonatal morbidity and mortality. Prophylactic use of probiotics has been suggested to protect preterm infants via supporting a healthy gut microbiota (GM) development, but the suggested strains and doses vary between studies. In this study, we profiled the GM of 5, 10 and 30-day fecal samples from two cohorts of preterm neonates (born <30 weeks of gestation) recruited in the same neonatal intensive care unit. One cohort (n = 165) was recruited from September 2006 to January 2009 before probiotics were introduced in the clinic. The second cohort (n = 87) was recruited from May 2010 to October 2011 after introducing Lacticaseibacillus rhamnosus GG and Bifidobacterium animalis ssp. lactis BB-12 supplementation policy. Through V3-V4 region 16S rRNA gene amplicon sequencing, a distinct increase of L. rhamnosus and B. animalis was found in the fecal samples of neonates supplemented with probiotics. During the first 30 days of life, the preterm GM went through similarly patterned progression of bacterial populations. Staphylococcus and Weissella dominated in early samples, but was gradually overtaken by Veillonella, Enterococcus and Enterobacteriaceae. Probiotic supplementation was associated with pronounced reduction of Weissella, Veillonella spp. and the opportunistic pathogen Klebsiella. Potential nosocomial pathogens Citrobacter and Chryseobacterium species also gradually phased out. In conclusion, probiotic supplementation to preterm neonates affected gut colonization by certain bacteria, but did not change the overall longitudinal bacterial progression in the neonatal period.
Abbreviations: GM: Gut microbiota; ASV: Amplicon sequence variant; NEC: Necrotizing enterocolitis; DOL: Days of life; NICU: Neonatal intensive care unit; ESPGHAN: European Society for Pediatric Gastroenterology, Hepatology and Nutrition; Db-RDA: Distance-based redundancy analysis; PERMANOVA: Permutational multivariate analysis of variance; ANCOM: Analysis of compositions of microbiomes; LGG: Lacticaseibacillus (former Lactobacillus) rhamnosus GG; BB-12: Bifidobacterium animalis ssp. lactis BB-12; DGGE: Denaturing Gradient Gel Electrophoresis</abstract><cop>United States</cop><pub>Taylor & Francis</pub><pmid>34264803</pmid><doi>10.1080/19490976.2021.1951113</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-7536-3453</orcidid><orcidid>https://orcid.org/0000-0001-6227-9906</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | amplicon sequencing Bifidobacterium animalis - isolation & purification Cohort Studies Denmark Enterocolitis, Necrotizing - drug therapy Enterocolitis, Necrotizing - immunology Feces - microbiology Female Gastrointestinal Microbiome - drug effects Gastrointestinal Microbiome - immunology gut microbiome Humans Infant, Newborn Infant, Newborn, Diseases - drug therapy Infant, Newborn, Diseases - immunology Infant, Premature - growth & development Infant, Premature - immunology Lactobacillus - isolation & purification Male necrotizing enterocolitis Preterm probiotics Probiotics - pharmacology Probiotics - therapeutic use Research Paper |
| title | The effect of early probiotic exposure on the preterm infant gut microbiome development |
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