Effect of linoleic acid supplementation on triglyceride content and gene expression in milk fat synthesis in two- and three-dimensional cultured bovine mammary epithelial cells

In this study, the effect of 0, 40, and 80 μmol/L of LA on triglyceride (TG) content was evaluated, as well as the mRNA expression of genes involved in milk fat synthesis in bovine mammary epithelial cells (BMECs) which were cultured in two-dimensional (2D) and three-dimensional (3D) models. TG cont...

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Veröffentlicht in:	Italian journal of animal science 2018-07, Vol.17 (3), p.714-722
Hauptverfasser:	Li, Dabiao, Xing, Yuanyuan, Li, Honglei, Wang, Weiyun, Hou, Xianzhi, Gao, Min
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetyl-CoA carboxylase Acyltransferase bovine mammary epithelial cells Cell culture Desaturase Diglycerides Epithelial cells Fatty acid-binding protein Fatty acids Fatty-acid synthase Gene expression Linoleic acid Mammary gland Milk milk fat genes Oils & fats Peroxisome proliferator-activated receptors Regulatory sequences Stearoyl-CoA desaturase Supplements three-dimensional culture model triglyceride
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Zusammenfassung:	In this study, the effect of 0, 40, and 80 μmol/L of LA on triglyceride (TG) content was evaluated, as well as the mRNA expression of genes involved in milk fat synthesis in bovine mammary epithelial cells (BMECs) which were cultured in two-dimensional (2D) and three-dimensional (3D) models. TG content was measured with a TG determination kit, and the expression of genes was measured with real-time quantitative polymerase chain reactions (RT-qPCR). Adding 80 μmol/L of LA significantly increased intracellular TG content in both 2D and 3D cultured BMECs (p
ISSN:	1828-051X 1594-4077 1828-051X
DOI:	10.1080/1828051X.2017.1412273