Green synthesis of silver nanoparticles mediated by Daucus carota L.: antiradical, antimicrobial potentials, in vitro cytotoxicity against brain glioblastoma cells

Green synthesis is a more eco-friendly approach compared to the chemical and physical methods. This simple green eco-friendly method was utilized to obtain silver nanoparticles mediated by an aqueous leaf extract as a precursor. The silver nanoparticles mediated by an aqueous solution of Daucus carota L. leaf extract (DCLE) as bio-reducer and stabilizer agents are described. The AgNPs were characterized using FT-IR, UV-Vis, PXRD, and TEM. Antimicrobial activities of the samples were investigated against drug-resistant bacterial isolates using the disk diffusion method, while in vitro cytotoxicity assay by MTS end-point and xCELLigence real-time assays. The synthesized AGNPs from DCLE exhibited face-centered cubic phase with average particle sizes of 18.26 (±6.86) nm and 16.81 (±7.72) nm for AgNP05 and AgNP01 respectively. The scavenging ability of DPPH radicals by the nanoparticles was studied for their antioxidant potentials. The study revealed that the compounds are capable of scavenging DPPH radicals in a dose-dependent pattern (IC 50 = 2.61 ± 0.58 μM for extract, 2.79 ± 1.75 μM for AgNP05, 2.84 ± 0.96 μM for AgNP01). Antimicrobial results showed that the nanoparticles possess higher activities. Cytotoxicity of the nanoparticles (0.00 μg/ml - 100 μg/ml) against U87MG brain glioblastoma cells, showed ED 50 = 87.31 and 109.48 μg/ml.