Optimization of supercritical fluid extraction of geniposidic acid from plantain seeds using response surface methodology

The process parameters of supercritical CO 2 (SCCO 2 ) plus modifer for the extraction of geniposidic acid from plantain seeds were studied using a Box-Behnken design. The effects of independent variables, that is, ethanol concentration (0-70%, ethanol:water, v/v), extraction pressure (10-30 MPa), and temperature (50-80°C) on the yield of geniposidic acid were evaluated. Results indicated that the data could be well fitted to a second-order polynomial model. The effects of ethanol concentration and temperature, as well as the interaction between ethanol concentration and temperature were significant (p < 0.05). The yield (8.9 mg/g) of modified SCCO 2 extraction at optimal conditions was compared with that obtained by Soxhlet extraction or ultrasound assisted extraction.