Synthesis of Triple Helix Forming Oligonucleotides with a Stretched Phosphodiester Backbone

Synthesis of Triple Helix Forming Oligonucleotides with a Stretched Phosphodiester Backbone

Total synthesis of novel DMT-phosphoramidites of thymidine (11 and 15) and 2′-deoxyguanosine (8 and 20) have been accomplished. The utility of these modified building blocks in the preparation of triple helix forming oligodeoxyribonucleotides with a stretched phosphodiester backbone has been evaluat...

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Veröffentlicht in:Nucleosides & nucleotides 1994-03, Vol.13 (1-3), p.255-273
Hauptverfasser: Sudhakar Rao, T., Jayaraman, Krishna, Durland, Ross H., Revankar, Ganapathi R.
Format: Artikel
Sprache:eng
Schlagworte:
Carbohydrates. Nucleosides and nucleotides
Chemistry
Exact sciences and technology
Nucleosides, nucleotides and oligonucleotides
Organic chemistry
Preparations and properties
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Zusammenfassung:Total synthesis of novel DMT-phosphoramidites of thymidine (11 and 15) and 2′-deoxyguanosine (8 and 20) have been accomplished. The utility of these modified building blocks in the preparation of triple helix forming oligodeoxyribonucleotides with a stretched phosphodiester backbone has been evaluated. It was found that the oligonucleotides with extended backbones were unable to enhance the binding to duplex targets containing CG or TA base pairs.
ISSN:0732-8311
2332-3892
DOI:10.1080/15257779408013239