Effect of Explant Source on in Vitro Propagation of Paulownia Tomentosa Steud
Shoot tip, leaf with petiole, nodal stem and root explants of in vitro germinated seedlings of Paulownia tomentosa Steud. were cultured on Murashige and Skoog (MS) medium combined with 1-naphthaleneaceticacid (NAA)/6-benzylaminopurine (BAP), indole3-aceticacid (IAA)/BAP or IAA/Kinetin. Nodal stem ex...
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Veröffentlicht in: | Biotechnology, biotechnological equipment biotechnological equipment, 2005-01, Vol.19 (3), p.20-26 |
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Sprache: | eng |
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Zusammenfassung: | Shoot tip, leaf with petiole, nodal stem and root explants of in vitro germinated seedlings of Paulownia tomentosa Steud. were cultured on Murashige and Skoog (MS) medium combined with 1-naphthaleneaceticacid (NAA)/6-benzylaminopurine (BAP), indole3-aceticacid (IAA)/BAP or IAA/Kinetin. Nodal stem explants were found to be an excellent explant source to induce direct ogranogenesis. The highest number of shoot regeneration from stem explants was obtained on MS medium supplemented with 0.1 mg.l
−1
IAA+3.0 mg.l
−1
BAP. Rooting was induced on MS basal or MS containing NAA+IAA combinations. The rooted plantlets were transferred to potting soil and adopted to greenhouse conditions. |
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ISSN: | 1310-2818 1314-3530 |
DOI: | 10.1080/13102818.2005.10817222 |