Enantiomer Separation by HPLC on Reversed Phase Silica Gel Coated with Copper (II) Complexes of (R,R)-Tartaric Acid Mono-Amide Derivatives

Two chiral ligand exchange stationary phases for HPLC, (R,R)-tartaric acid mono-(R)-1-(α-naphthyl)ethylamide [I] and (R,R) -tartaric acid mono-n-laurylamide [11], were prepared. The direct separation of a number of amino acid, hydroxy acid and amino alcohol enan-tiomers was achieved using ODS silica gel coated with [I] and [11], and water or hydro-organic eluents containing copper(ll) ion as a mobile phase. The enantioselectivity of the phase [I] was superior than that of the phase [11]. The phase [I] is very promisir.-as the chiral coating agent on reversed phase materials for the direct separation of a variety of enantiomers by ligand exchange HPLC.