Development of an antibody-based colloidal gold immunochromatographic lateral flow strip test for natamycin in milk and yoghurt samples

An immunochromatographic lateral flow strip test was developed for the detection of natamycin (Nata) residues in milk and cheese samples. Monoclonal antibody (mAb) against Nata was produced with a half maximal inhibitory concentration of 1.85 μg L −1 . MAb conjugated with gold nanoparticles was the...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Food and agricultural immunology 2017-11, Vol.28 (6), p.1283-1292
Hauptverfasser: Liu, Liqiang, Chen, Yanni, Song, Shanshan, Zheng, Qiankun, Wu, Xiaoling, Kuang, Hua
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:An immunochromatographic lateral flow strip test was developed for the detection of natamycin (Nata) residues in milk and cheese samples. Monoclonal antibody (mAb) against Nata was produced with a half maximal inhibitory concentration of 1.85 μg L −1 . MAb conjugated with gold nanoparticles was the detection reagent. Nata conjugated with ovalbumin via the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide method immobilized on a nitrocellulose membrane was the capture reagent. This semi-quantitative method required only 15 min to complete. The optimum concentrations of coating antigen and mAb were 0.15 µg mL −1 and 0.2 µg mL −1 , respectively. Based on an optical density scanner, the visual limit of detection of Nata was 5 μg L −1 and 10 μg kg −1 in milk and yoghurt samples, respectively.
ISSN:0954-0105
1465-3443
DOI:10.1080/09540105.2017.1337084