A CRISPR/Cas9-mediated gene knockout system in Aspergillus luchuensis mut. kawachii

We developed an approach to genome editing of the white koji fungus, Aspergillus luchuensis mut. kawachii using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system. Co-transformation of AMA1-based Cas9 and gRNA expression plasmids achieved efficient gene knockout in A. kaw...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2020-10, Vol.84 (10), p.2179-2183
Hauptverfasser:	Kadooka, Chihiro, Yamaguchi, Masaaki, Okutsu, Kayu, Yoshizaki, Yumiko, Takamine, Kazunori, Katayama, Takuya, Maruyama, Jun-Ichi, Tamaki, Hisanori, Futagami, Taiki
Format:	Artikel
Sprache:	eng
Schlagworte:	AMA1 Aspergillus - genetics Aspergillus luchuensis mut. kawachii Base Sequence CRISPR-Cas Systems - genetics CRISPR/Cas9 Gene Knockout Techniques - methods genome editing Mutation shochu
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Beschreibung
Zusammenfassung:	We developed an approach to genome editing of the white koji fungus, Aspergillus luchuensis mut. kawachii using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system. Co-transformation of AMA1-based Cas9 and gRNA expression plasmids achieved efficient gene knockout in A. kawachii. The plasmids were easily lost when selective pressure was removed, allowing for successive rounds of genome editing.
ISSN:	0916-8451 1347-6947
DOI:	10.1080/09168451.2020.1792761