An Improved Deprotection Procedure of Amine-Containing Oligonucleotides from Acrylonitrile Modification

An Improved Deprotection Procedure of Amine-Containing Oligonucleotides from Acrylonitrile Modification

Alkylation of alkylamine by acrylonitrile via Michael addition 1 occurred when standard ammonium hydroxide deprotection was used. The alkylation was greatly reduced using an improved two-step procedure when BCE phosphotriester protecting groups were selectively removed with t-butylamine prior to amm...

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Veröffentlicht in:Nucleosides & nucleotides 1999-06, Vol.18 (6-7), p.1205-1206
Hauptverfasser: Chang, Chu-An, Horn, Thomas
Format: Artikel
Sprache:eng
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Zusammenfassung:Alkylation of alkylamine by acrylonitrile via Michael addition 1 occurred when standard ammonium hydroxide deprotection was used. The alkylation was greatly reduced using an improved two-step procedure when BCE phosphotriester protecting groups were selectively removed with t-butylamine prior to ammonium hydroxide deprotection.
ISSN:0732-8311
DOI:10.1080/07328319908044663