High Performance Liquid Chromatographic Separation of Bacitracin Methylenedisalicylate

Our previously described isocratic RP HPLC procedure was convenient for monitoring and quality control of bacitracin production and Zn - bacitracin feed grade preparations. Separation and quantitative determination of the main active bacitracin components (A 1 B 1 and B 2 ) are possible and those elution is not interrupted by other ingredients in this type of samples. But when the methylenedisalicylic salt of bacitracin was tested some modification of method were necessary for the correct separation of bacitracin components. Mobile phase had to be modified and polystyrene based packing was an alternative and useful complement to octadecylated silica gel packings.