HPLC Purification of Solid-Phase Generated Synthetic Bombesin

A scheme based on ion-exchange and reverse-phase high pressure liquid chromatography has been utilized for the semi-preparative and preparative purification of the solid phase generated model peptide bombesin. The final product showed a purity ≥ 99% in analytical reverse-phase high pressure liquid c...

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Veröffentlicht in:	Journal of liquid chromatography 1990-09, Vol.13 (16), p.3289-3311
Hauptverfasser:	Bello, Carlo Di, Lucchiari, Adriana, Buso, Orfeo, Coldani, Roberto
Format:	Artikel
Sprache:	eng
Schlagworte:	Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Biological and medical sciences Fundamental and applied biological sciences. Psychology Proteins
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A scheme based on ion-exchange and reverse-phase high pressure liquid chromatography has been utilized for the semi-preparative and preparative purification of the solid phase generated model peptide bombesin. The final product showed a purity ≥ 99% in analytical reverse-phase high pressure liquid chromatography and was identical to authentic bombesin as demonstrated by different physico-chemical and biological criteria. The results are discussed and compared to those obtained using countercurrent chromatography.
ISSN:	0148-3919 2331-0413
DOI:	10.1080/01483919008049102